Unit 7, No. 52, Western Mansouri street, ShahrAra, Tehran Iran

Office Address

Unit 7, No. 52, Western Mansouri street, ShahrAra, Tehran Iran

Email Address

info@arian-cell.com

RiboGrip™ RNase Inhibitor

  • fully active at a wide range of temperature from 37°C to 50°C
  • included in our cDNA synthesis kits and 1-step RT-qPCR kits
  • reaction set-up and shipment without ice

Description

RiboGrip™ RNase Inhibitor is an in silico-designed protein-based ribonuclease inhibitor, which inactivates RNase A.

RiboGrip™ inhibits the activity of ribonuclease A by noncovalently binding in a non-competitive mode at a ratio of 1:1. This novel inhibitor  has enhanced stability at room temperature with no loss of activity for up to 1 month.

Applications

Preventing RNA degradation in the following applications:

  • First-strand cDNA synthesis
  • RT-PCR
  • RT-qPCR
  • in vitro transcription and translation

Additional information

Routine storage: -20ºC

Properties

Concentration: 40 U/µl

Source: Purified from an E.coli strain that carries an overproducing plasmid containing a RiboGrip™ RNase Inhibitor gene.

Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 5 mM DTT and stabilizers.

Recommendations for use: RiboGrip RNase Inhibitor™ is used generally at a final reaction concentration of 1 U/μl. Inactivated by heating at 85°C for 5 min.

Included in FIREScript® and SOLIScript® cDNA Synthesis Kits and Mixes, and in all SOLIScript® 1-step RT-PCR kits

Related Products

SOLIScript® 1-st...

Convenient kit format for performing highly specific cDNA synthesis and probe-ba...

SOLIScript® KIT

Novel in silico-engineered, thermostable reverse transcriptase with reduced...

FIREScript KIT

Genetically modified MMLV-based reverse transcriptase with increased thermostab...