Description
SOLIScript® 1-step CoV Kit is optimized for highly sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA by one-step real-time RT-PCR (RT-qPCR).
The Kit is suitable for the detection of up to four targets simultaneously and can be used with all common qPCR cyclers. Follow recommendations specific for a selected SARS-CoV-2 diagnostic panel, and instrument-specific guidelines. Contact us if a passive reference dye is required for signal normalization.
SARS-CoV-2 primers and probes, applicable positive and internal extraction controls are not provided with the Kit and should be supplied by the user.
SOLIScript® 1-step CoV Kit is tested and validated for SARS-CoV-2 detection by diagnostic laboratories. Please see our recommendations for US-CDC and Berlin-Charité protocols.
Note! Amplification of more than one target in a single reaction may require considerable optimization, individual assays must be validated prior to multiplexing. For multiplex reactions, design primer pairs with similar annealing temperatures for all targets to be amplified. Self-complementarity of primers should be avoided.
Applications
Detection and quantification of RNA targets with qPCR (including SARS-CoV-2)
DNA/LNA hydrolysis probe-based assays
Suitable for singleplex and multiplex reactions