Unit 7, No. 52, Western Mansouri street, ShahrAra, Tehran Iran

Office Address

Unit 7, No. 52, Western Mansouri street, ShahrAra, Tehran Iran

Email Address

info@arian-cell.com

SOLIScript® 1-step CoV Kit

Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip™ RNase inhibitor, and nuclease-free water.

  • Optimized for SARS-CoV-2 detection
  • Flexible assay set-up
  • Suitable for multiplexing (up to 4 targets in a single assay)
  • Reaction set-up and shipment without ice
  • RNase inhibitor included in the kit

Description

SOLIScript® 1-step CoV Kit is optimized for highly sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA by one-step real-time RT-PCR (RT-qPCR).

The Kit is suitable for the detection of up to four targets simultaneously and can be used with all common qPCR cyclers. Follow recommendations specific for a selected SARS-CoV-2 diagnostic panel, and instrument-specific guidelines. Contact us if a passive reference dye is required for signal normalization.

SARS-CoV-2 primers and probes, applicable positive and internal extraction controls are not provided with the Kit and should be supplied by the user.

SOLIScript® 1-step CoV Kit is tested and validated for SARS-CoV-2 detection by diagnostic laboratories. Please see our recommendations for US-CDC and Berlin-Charité protocols. 

Note! Amplification of more than one target in a single reaction may require considerable optimization, individual assays must be validated prior to multiplexing. For multiplex reactions, design primer pairs with similar annealing temperatures for all targets to be amplified. Self-complementarity of primers should be avoided.

Applications

Detection and quantification of RNA targets with qPCR (including SARS-CoV-2)

DNA/LNA hydrolysis probe-based assays

Suitable for singleplex and multiplex reactions

Additional information

Properties

Sample type: RNA

Product format: Reagent kit 

Detection type: Probe-based

Reference dye: not included 

Compatible real-time instruments: Most qPCR platforms. Check Your cycler!

Reagents 

5x One-step Probe CoV Mix  with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2)

40x One-step SOLIScript® CoV Mix with SOLIScript® Reverse Transcriptase and RiboGrip™ RNase Inhibitor 

Water, nuclease free

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