بزرگراه جلال آل احمد،خیابان آرش مهر،بلوار غربی،خیابان منصوری،پلاک 52،واحد 7
Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip™ RNase inhibitor, and nuclease-free water.
SOLIScript® 1-step CoV Kit is optimized for highly sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA by one-step real-time RT-PCR (RT-qPCR).
The Kit is suitable for the detection of up to four targets simultaneously and can be used with all common qPCR cyclers. Follow recommendations specific for a selected SARS-CoV-2 diagnostic panel, and instrument-specific guidelines. Contact us if a passive reference dye is required for signal normalization.
SARS-CoV-2 primers and probes, applicable positive and internal extraction controls are not provided with the Kit and should be supplied by the user.
SOLIScript® 1-step CoV Kit is tested and validated for SARS-CoV-2 detection by diagnostic laboratories. Please see our recommendations for US-CDC and Berlin-Charité protocols.
Note! Amplification of more than one target in a single reaction may require considerable optimization, individual assays must be validated prior to multiplexing. For multiplex reactions, design primer pairs with similar annealing temperatures for all targets to be amplified. Self-complementarity of primers should be avoided.
Detection and quantification of RNA targets with qPCR (including SARS-CoV-2)
DNA/LNA hydrolysis probe-based assays
Suitable for singleplex and multiplex reactions
Sample type: RNA
Product format: Reagent kit
Detection type: Probe-based
Reference dye: not included
Compatible real-time instruments: Most qPCR platforms. Check Your cycler!
5x One-step Probe CoV Mix with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2)
40x One-step SOLIScript® CoV Mix with SOLIScript® Reverse Transcriptase and RiboGrip™ RNase Inhibitor
Water, nuclease free
